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2 edition of Recombinant vaccinia viruses in the analysis of human cytotoxic T-cell function found in the catalog.

Recombinant vaccinia viruses in the analysis of human cytotoxic T-cell function

Heather Griffin

Recombinant vaccinia viruses in the analysis of human cytotoxic T-cell function

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Published by University of Birmingham in Birmingham .
Written in English


Edition Notes

Thesis (Ph.D) - University of Birmingham, Dept of Cancer Studies.

Statementby Heather Griffin.
ID Numbers
Open LibraryOL20860544M

Objective: We sought to characterize virus specific cytotoxic T (Tc) and T helper (Th) cells in 28 AD subjects, of which 14 had a history of EH. Methods: HSV1‐ and influenza‐specific Tc and Th cells were analyzed by means of reactive T cell lines, as well as the activation markers CD (CD40L) and CD (4‐1BB), and MHC‐tetramers. Antibody to human T cell leukaemia virus type III in Australian homosexual men with lymphadenopathy. Frazer, I.H., Sarngadharan, M.G., Mackay, I.R. and Gallo, R.C. () Antibody to human T cell leukaemia virus type III in Australian homosexual men with lymphadenopathy. Medical Journal of Australia, 5:   The durability of T-cell memory is explored in the context of several acute viral infections including vaccinia virus (VV), measles virus (MV) and yellow fever virus (YFV). Following acute infection, different virus-specific T-cell subpopulations exhibit distinct cytokine profiles and these profiles change over the course of infection. Immunobiology of cytotoxic T-cell resistant variants: studies on lymphocytic choriomeningitis virus (LCMV) Semin Virol. Recombinant vaccinia viruses and the development of immunization strategies using influenza virus. J Infect Dis. The human cytotoxic T cell response to influenza A vaccination. Clin Exp by:


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Recombinant vaccinia viruses in the analysis of human cytotoxic T-cell function by Heather Griffin Download PDF EPUB FB2

Biddison WE, Sharrow SO, Shearer GM () T cell subpopulations required for the human cytotoxic T lymphocyte response to influenza virus: evidence for T cell help. J Cited by: Wei Chen, Angela Wandinger-Ness, in Methods in Enzymology, Biochemical Analyses Rab8 and Rab11a Functions in Exocytosis Monitored by Cell Surface Biotinylation.

A recombinant vaccinia virus expression system was used to establish a biochemical assay useful for studying the function of Rab proteins in exocytic transport. This system was utilized on account of several important advantages.

We evaluated a DNA plasmid-vectored vaccine and a recombinant modified vaccinia virus Ankara vaccine (MVA-mBN32), each encoding cytotoxic and helper T-lymphocyte epitopes of human immunodeficiency virus type 1 (HIV-1) in a randomized, double-blinded, placebo-controlled trial in 36 HIVuninfected adults using a heterologous prime-boost by: We constructed recombinant MVA viruses expressing the human tyrosinase gene under the control of the early / late vaccinia virus promoter Grant P Recently human tyrosinase was identified as a melanoma-specific tumor antigen, which allows the formation of anti-tumor cytolytic T lymphocytes (Brichard, V.

et al. [] J. Exp. Med.).Author: Gerd Sutter, Marion Ohlmann, Volker Erfle. The chapter presents basic concepts and applications of poxviruses as recombinant expression vectors. Additional focused reviews on poxvirus molecular biology construction of recombinant poxviruses and their applications as vaccine vectors for infectious diseases and cancer immunotherapy Vaccinia virus (VV), along with smallpoxvirus, is a member of the orthopoxvirus genus, within the family Cited by: 3.

In some cases, DNA vaccines have proven more immunogenic than other recombinant delivery systems (for example, recombinant vaccinia viruses, see ref. 99), and sometimes they appear to overcome a host's previous nonresponsiveness to a particular antigen. As a rule, DNA vaccines appear to induce better CD8 T cell responses than antibody Cited by: 3.

Recombinant vaccinia viruses in the analysis of human cytotoxic T-cell function book As a research tool, recombinant vaccinia viruses are used to synthesize and analyze the structure-function relationships of proteins, determine Recombinant vaccinia viruses in the analysis of human cytotoxic T-cell function book targets of humoral and cell-mediated immunity.

Recombinant vaccinia viruses (W) were propagated as described (30,31). The production and characterization of W expressing influenza virus nucleoprotein (NP). The present invention relates to recombinant vaccinia viruses derived from the modified vaccinia virus Ankara (MVA) and containing and capable of expressing foreign genes which are inserted at the site of a naturally occurring deletion in the MVA genome, and the use of such recombinant MVA viruses for the production of polypeptides, e.g.

antigens or therapeutic agents, or viral vectors for Cited by: Respiratory viruses infections caused by influenza viruses, human parainfluenza virus (hPIV), respiratory syncytial Recombinant vaccinia viruses in the analysis of human cytotoxic T-cell function book (RSV) and coronaviruses are an eminent threat for public health.

Currently, there are no licensed vaccines available for hPIV, RSV and coronaviruses, and the available seasonal influenza vaccines have considerable limitations.

With regard to pandemic preparedness, it is Cited by: Costimulatory factor OXligand (OX40L) plays a crucial role in the T cell-dependent humoral immune responses through T-B cell interaction.

In this work, a recombinant RABV overexpressing mouse OX40L (LBNSE-OX40L) was constructed, and its effects on immunogenicity were evaluated in a mouse model. Coronaviruses represent a major group of viruses of both molecular biological interest and clinical significance in animals and humans. During the past two decades, coronavirus research has been an expanding field and, sincean international symposium was held every 3 years.

We organized theBrand: Springer US. Lastly, some recombinant viruses can be lyophilized and stored without the need for special refrigeration equipment. Recombinant vaccinia viruses in the analysis of human cytotoxic T-cell function book the recombinant viruses most commonly used as vaccine vectors, there are already established high-throughput and large-scale production processes, aiming to use this technology in the context of : Dulcilene Mayrink de Oliveira, Jonatan Marques Campos, Soraia de Oliveira Silva, Maria Norma Melo.

A vaccinia recombinant expressing the rabies virus glycoprotein was an early example of a successful pox virus vector useful in immunization ().The vector was constructed by the insertion of the encoding cDNA for the rabies virus glycoprotein in the thymidine kinase locus of the Copenhagen strain of.

Yoshida, M., Sciki, M., Yamaguchi, K., and Takatsuki, K.,Monoclonal integration of human T-cell leukemia provirus in all primary tumors of adult T-cell leukemia suggests causative role of human T-cell leukemia virus in disease, Proc.

Natl. Acad. Sci. USA – PubMed Google ScholarCited by:   Human carcinoembryonic antigen (CEA) is a well-characterized oncofetal glycoprotein whose overexpression by human carcinomas has been a target for cancer immunotherapy.

Transgenic mice that express CEA as a self-antigen with a tissue distribution similar to that of humans have been developed. This study investigates: (a) the responsiveness of the CEA transgenic () mice to Cited by: Sequence analysis shows it to be a processed form of a polypeptide encoded in the vaccinia virus genome which is related to epidermal growth factor (EGF) and α-transforming growth factor (aTGF).

The amino terminus of the processed vaccinia virus growth factor (VVGF) begins at residue 20 of the primary translation product, indicating a signal. Human T-cell leukemia virus type I (HTLV-I) is implicated in the pathogenesis of adult T-cell leukemia/lymphoma (ATL), HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP), and other inflammatory diseases (1–5).A viral regulatory protein, HTLV-I Tax, is known to have multiple functions responsible for activation of cells and is capable of immortalizing rat and human cells in Cited by: Among animal viruses, DNA-containing viruses were the first to become amenable to genetic engineering techniques.

This breakthrough was achieved for simian virus 40 when a cloned cDNA copy was transfected into cells, resulting in the formation of infectious virus (see Table 1).Transfected mutated cDNA molecules gave rise to defined mutant viruses (). Recombinant vaccinia viruses which express MHV-JHM proteins: protective immune response and the influence of vaccination on coronavirus-induced encephalomyelitis Characterization of human T cell clones specific for coronavirus E.

Molecular analysis. Types of interferon. Based on the type of receptor through which they signal, human interferons have been classified into three major types. Interferon type I: All type I IFNs bind to a specific cell surface receptor complex known as the IFN-α/β receptor that consists of IFNAR1 and IFNAR2 chains.

The type I interferons present in humans are IFN-α, IFN-β, IFN-ε, IFN-κ and ro: IPR such as viruses and bacteria, detect dangers, and initiate immune responses.

Immature patrolling dendritic cells (DCs) have high endocytic activity and a low T-cell activation potential. Contact with a pathogen induces maturation and the expression of certain cell-surface molecules, greatly enhancing their ability to activate T Size: KB.

History. The adeno-associated virus (AAV), previously thought to be a contaminant in adenovirus preparations, was first identified as a dependoparvovirus in the s in the laboratories of Bob Atchison at Pittsburgh and Wallace Rowe at gical studies in humans subsequently indicated that, despite being present in people infected by helper viruses such as adenovirus or herpes virus Class: incertae sedis.

Principles o Vaccination 1 1 Immunology and Vaccine-Preventable Diseases Immunology is a complicated subject, and a detailed. discussion of it is beyond the scope of this text.

However, an understanding of the basic function of the immune system is useful in order to understand both how vaccines work and the basis of recommendations for their Size: 97KB. Research on arenaviruses holds promise for elucidating basic mechanisms of immunological tolerance, viral immunosuppression, the nature of protective immune responses to vaccination, and viral effects on cell function.

This volume presents the current molecular, organismal, and epidemiological resea. The year marks the centenary of Francis Peyton Rous's landmark experiments on an avian cancer virus.

Since then, seven human viruses have been found to cause 10–15% of human cancers by: TER94 constitutes an important AAA+ ATPase that associates with diverse cellular processes, including protein quality control, membrane fusion of the Golgi apparatus and endoplasmic reticulum network, nuclear envelope reformation, and DNA replication.

To date, little is known regarding the role (s) of TER94 in the baculovirus life cycle. Viruses are small (20–nm in diameter) protein packages, containing genetic material (DNA or RNA); some also contain enzymes. Viruses depend on living cells for their existence, genome expression, and replication.

They have colonized most life forms, including bacteria, plants, insects, and animals. High prevalence and mortality rates of cervical cancer create an imperative need to clarify the uniqueness of HPV (Human Papillomavirus) infection, which serves as the key causative factor in cervical malignancies.

Understanding the immunological details and the microenvironment of the infection can be a useful tool for the development of novel therapeutic by:   Shown are Vaccine-elicited CD4 + T cell responses (A) and CD8 + T-cell responses (B) before immunization and at weeks 2, 10, 17, 21, 25, and 37 in the trial.

Responses are shown as interferon (IFN)–γ secreting cells measured by intracellular cytokine staining (ICS) after Gag and Env peptide stimulation of peripheral blood mononuclear cells Cited by: Vaccination is one of the most successful approaches for controlling various viral diseases.

Novel approaches will be needed to develop highly effective vaccines to prevent infectious diseases such as HIV. There are many aspects of HIV-1 biology that make the development of an HIV vaccine difficult, including viral diversity, effective type of immune response, and suitable experimental model Cited by: 1.

The human cytotoxic T-lymphocyte (CTL) response to cytomegalovirus is dominated by structural protein pp frequency, specificity and T-cell-receptor usage of Cited by: Contact Information.

Surg Oncol Res Fac, Durham, NC Box Med Ctr, Durham, NC [email protected] () @article{osti_, title = {A fusion protein of HCMV IE1 exon4 and IE2 exon5 stimulates potent cellular immunity in an MVA vaccine vector}, author = {Wang, Z and Zhou, W and Srivastava, T and La Rosa, C and Mandarino, A and Division of Virology, Beckman Research Institute of the City of Hope, California and Forman, S J and Zaia, J A.

de Alencar BC, Persechini PM, Haolla FA, de Oliveira G, Silverio JC, et al. () Perforin and gamma interferon expression are required for CD4 + and CD8 + T-cell-dependent protective immunity against a human parasite, Trypanosoma cruzi, elicited by heterologous plasmid DNA prime-recombinant adenovirus 5 boost vaccination.

Infect Immun 77 Cited by: Primary liver cancer is a common kind of digestive cancers with high malignancy, causingdeaths each year. Hepatocellular carcinoma is the major pathological type of primary liver cancer.

Traditional treatment methods for patients with hepatocellular carcinoma have shown poor efficacy in killing residual cancer cells for a long time.

In recent years, tumor immunotherapy has emerged as Cited by: 9. For more than 20 years perforin and granzymes (GZMs) have been recognized as key cell death executors of cytotoxic T (Tc) and natural killer (NK) cells during cancer immunosurveillance.

In immune surveillance, perforin and GZMB, the most potent cytotoxic molecules, act mainly as antitumoral and anti-infectious factors.

However, when expressed by immune regulatory cells they may contribute to Cited by: 6. which is delivered through a modified Vaccinia virus, induces CD8 + T cell responses in a mouse model; they further identify a MERS-CoV N protein-specific CD8 + T cell epitope on the vaccine [40].

Overall, these reports demonstrate that a variety of promising vaccine tools are available to prevent MERS-CoV infection in humans and other animals. Herein, we show that a recombinant fusion protein of a humanized antibody to CD40 fused to HPVE6/7 (αCDHPVE6/7) can evoke HPVE6/7.

This strategy was used to characterize the Ig repertoire of plasma cells/plasmablasts in AHI and to produce recombinant influenza mAbs from sorted single human plasmablasts after influenza vaccination. The lab is also studying the earliest effect HIV-1 has on B cells.

The angstrom crystal structure of the human T cell receptor CD3E Gamma heterodimer pdf to the therapeutic MAB OKT3 L Kjer-Nielsen, MA Dunstone, L Kostenko, LK Ely, T Beddoe, N Mifsud, AW Purcell, AG Brooks, J Rossjohn, J McCluskey.Lag-3 has emerged as an important molecule in T cell biology.

We investigated the role of Lag-3 in conventional T cell (Tcon) and regulatory T cell (Treg) function in murine GVHD with the hypothesis that Lag-3 engagement diminishes alloreactive T cell responses after bone marrow transplantation. SUMMARY Before ebook emergence of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) inonly ebook other animal or human coronaviruses were known.

The discovery of this virus was soon followed by the discovery of the civet and bat SARS-CoV and the human coronaviruses NL63 and HKU1. Surveillance of coronaviruses in many animal species has increased Cited by: